Marie-Denise Villers (1774 – August 19, 1821) was a French painter, who specialized in portraits. She was born Marie-Denise Lemoine in Paris. She came from an artistic family, and her sisters Marie-Victoire Lemoine and Marie-Élisabeth Gabiou were also accomplished artists. In 1794, Marie-Denise married an architecture student, Michel-Jean-Maximilien Villers.
Villers was a student of the French painter Anne-Louis Girodet de Roussy-Trioson. She was first exhibited at the Paris Salon of the Year VII (1799). Villers' most famous painting, Young Woman Drawing, (1801) is displayed in the Metropolitan Museum of Art. The painting was attributed to Jacques-Louis David at one time, but was later realized to be Villers' work. It may be a self-portrait of the artist.